Normal prolactin levels in breastfeeding mothers

Typical baseline serum prolactin levels in women References
Not pregnant, not lactating < 25 ng/mL Walker p. 65, Riordan p. 76, Serri et al. p. 575
Pregnant, at term 200 ng/mL Walker p. 65
Lactating, 7 days postpartum 100 ng/mL Riordan p. 76
Lactating, 3 months postpartum 100 ng/mL Walker p. 65
Lactating, menstruation not started before 180 days 110 ng/mL Riordan p. 76
Lactating, menstruation started before 180 days 70 ng/mL Riordan p. 76
Lactating, 6 months postpartum 50 ng/mL Walker p. 65, Riordan p. 76

Per Riordan (2005, p. 76):

  • “Plasma prolactin levels increase the most in the immediate postpartum period but rise and fall in proportion to the frequency, intensity, and duration of nipple stimulation.”
  • ” Prolactin concentration in blood doubles in response to suckling and peaks approximately 45 minutes after the beginning of a breastfeeding session (Noel, Suh, and Frantz, 1974).”
  • “During the first week after birth, prolactin levels in breastfeeding women fall about 50 percent. If a mother does not breastfeed, prolactin levels usually reach nonpregnant levels by seven days postpartum (Tyson et al., 1972).”
  • Prolactin levels “follow a circadian rhythm: levels during the night (sleep) are higher than during the day.”
  • Prolactin levels “decline slowly over the course of lactation (Battin et al., 1985; Cox, Owens, & Hartmann, 1996) but remain elevated for as long as the mother breastfeeds, even if she breastfeeds for years (Stallings et al., 1996).”
  • Prolactin levels “rise with suckling: the more feedings, the higher the level of serum prolactin. More than eight breastfeedings per 24 hours prevents decline of the concentration of prolactin before the next breastfeeding (Cox, Owens, & Hartmann, 1996; Tay 1996).”

For women who have their prolactin levels checked, Burns & Haddad (1998) note: “When drawing blood for a prolactin level, caution should be used so as not to produce an artificial elevation. Since stress can elevate the prolactin level, the patient should rest (but not sleep) before the sample is obtained. Also, the level should not follow a breast examination and should be drawn in a fasting state.”

Related information

Galactorrhea (unexpected milk production) and other nipple discharge @ (Galactorrhea is sometimes associated with abnormally high prolactin levels.)


Battin DA, Marrs RP, Fleiss PM, Mishell DR Jr. Effect of suckling on serum prolactin, luteinizing hormone, follicle-stimulating hormone, and estradiol during prolonged lactation. Obstet Gynecol. 1985 Jun;65(6):785-8.

Cox DB, Owens RA, Hartmann PE. Blood and milk prolactin and the rate of milk synthesis in women. Exp Physiol. 1996 Nov;81(6):1007-20.

Ellison, PT. Breastfeeding, Fertility, and Maternal Condition. In: Stuart-Macadam P, Dettwyler KA, ed. Breastfeeding: Biocultural Perspectives. Hawthorne, NY: Aldine de Gruyter, 1995:305-345. (note Figure 11.2 on p. 313)

Lawrence R, Lawrence R. Breastfeeding: A Guide for the Medical Profession, 6th ed. Philadelphia, Pennsylvania: Mosby, 2005: 79-80,737-738.

Noel GL, Suh HK, Frantz AG. Prolactin release during nursing and breast stimulation in postpartum and nonpostpartum subjects. J Clin Endocrinol Metab. 1974 Mar;38(3):413-23.

Riordan J. Breastfeeding and Human Lactation, 3rd ed. Boston and London: Jones and Bartlett, 2005: 75-77.

Serri O, Chik CL, Ur E, Ezzat S. Diagnosis and management of hyperprolactinemia. CMAJ. 2003 Sep 16;169(6):575-81.

Stallings JF, Worthman CM, Panter-Brick C, Coates RJ. Prolactin response to suckling and maintenance of postpartum amenorrhea among intensively breastfeeding Nepali women. Endocr Res. 1996 Feb;22(1):1-28.

Tay CC, Glasier AF, McNeilly AS. Twenty-four hour patterns of prolactin secretion during lactation and the relationship to suckling and the resumption of fertility in breast-feeding women. Hum Reprod. 1996 May;11(5):950-5.

Tyson JE, Hwang P, Guyda H, Friesen HG. Studies of prolactin secretion in human pregnancy. Am J Obstet Gynecol. 1972 May 1;113(1):14-20.

Walker M. Breastfeeding Management for the Clinician: Using the Evidence. Boston: Jones and Bartlett, 2006: 63-66.

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